Redd1 knockdown prevents doxorubicin-induced cardiac senescence
Pianpian Huang, Lijuan Bai, Lihua Liu, Jun Fu, Kefei Wu, Hongxia Liu, Yun Liu, Benming Qi, Benling Qi
Abstract
. Notably, AAV9-shRNA-mediated Redd1 silencing significantly alleviated Dox-induced cardiac dysfunction. Moreover, through pharmacological inhibition, immunofluorescence, and western blotting, signaling pathway analyses indicated that Redd1 promotes cardiomyocyte senescence as a downstream effector of p38 MAPK to promote NF-kB signaling via p65 phosphorylation and nuclear translocation.
Topics & Concepts
Gene knockdownGene silencingSenescenceSmall hairpin RNACell biologyBiologyIn vivoDNA damageBlotMolecular biologyCancer researchCell cultureGeneGeneticsDNATelomeres, Telomerase, and SenescenceMitochondrial Function and PathologyPlant Molecular Biology Research