A metabolic regulatory network for the Caenorhabditis elegans intestine
Sushila Bhattacharya, Brent B. Horowitz, Jingyan Zhang, Xuhang Li, Hefei Zhang, Gabrielle E. Giese, Amy D. Holdorf, Albertha J.M. Walhout
Abstract
by performing RNAi of more than 1,400 metabolic genes with a set of 19 promoter reporter strains that express a fluorescent protein in the animal's intestine. We find that metabolic perturbations generally increase promoter activity, which contrasts with transcription factor (TF) RNAi, which tends to repress promoter activity. We identify several TFs that modulate promoter activity in response to perturbations of the electron transport chain and explore complex genetic interactions among metabolic pathways. This work provides a blueprint for a systems-level understanding of how metabolism affects gene expression.