Sanger Tree of Life Sample Preparation: Triage and Dissection v1
J.M. Jay, Halyna Yatsenko, Juan Pablo Narváez-Gómez, Haddijatou Mbye, Maria Morra, Michelle Strickland, Clare Cornwell, Caroline Howard
Abstract
This protocol describes the process of sample preparation for the extraction of DNA and/or RNA from the wide variety of samples processed by the Tree of Life Core Laboratory as part of the Tree of Life Programme. It also describes the triage steps and recommended weight requirements for the different taxonomic groups covered by the Tree of Life programme. The output of this protocol is a sufficient amount of sample that can be directed towards any of the Sanger Tree of Life Sample Homogenisation protocols.
Topics & Concepts
TriageTree of life (biology)Protocol (science)Tree (set theory)Sample (material)Computer scienceComputational biologyMedicineBiologyChromatographyChemistryMathematicsMedical emergencyPhylogenetic treePathologyGeneticsGeneAlternative medicineMathematical analysisForensic and Genetic ResearchMolecular Biology Techniques and ApplicationsGenetics, Bioinformatics, and Biomedical Research