Self-assembly of DNA nanogels with endogenous microRNA toehold self-regulating switches for targeted gene regulation therapy
Jiaqi Yan, Haixia Zou, Wenhui Zhou, Xiaowan Yuan, Zhijun Li, Xiaodong Ma, Chang Liu, Yonghui Wang, Jessica M. Rosenholm, Wenguo Cui, Xiangmeng Qu, Hongbo Zhang
Abstract
Herein, a smart nanohydrogel with endogenous microRNA-21 toehold is developed to encapsulate gemcitabine-loaded mesoporous silica nanoparticles for targeted pancreatic cancer therapy. This toehold mediated strand displacement method can simultaneously achieve specific drug release and miRNA-21 silencing, resulting in the up-regulation of the expression of tumor suppressor genes PTEN and PDCD4.
Topics & Concepts
microRNAEndogenyDNACell biologyChemistryGeneGenetic enhancementComputational biologyNanotechnologyBiologyBiochemistryMaterials scienceAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryMicroRNA in disease regulation