Biosynthesis of pleuromutilin congeners using an <i>Aspergillus oryzae</i> expression platform
Fabrizio Alberti, Khairunisa Khairudin, J. Davies, Suphattra Sangmalee, Christine L. Willis, Gary D. Foster, Andy M. Bailey
Abstract
Three novel pleuromutilin congeners were isolated, and their antimicrobial activity was investigated, alongside that of an additional derivative produced through a semi-synthetic approach. It was observed that the absence of various functional groups - 3 ketone, 11 hydroxyl group or 21 ketone - from the pleuromutilin framework affected the antibacterial activity of pleuromutilin congeners. This study expands our knowledge on the biosynthesis of pleuromutilin and provides avenues for the development of novel pleuromutilin analogues by combining synthetic biology and synthetic chemistry.
Topics & Concepts
BiosynthesisNatural productAspergillus oryzaeAntimicrobialBiologyChemistryBiochemistryStereochemistryMicrobiologyGeneFermentationVeterinary medicine and infectious diseasesMycobacterium research and diagnosisToxin Mechanisms and Immunotoxins