Receptor Switching in Newly Evolved Adeno-associated Viruses
L. Patrick Havlik, Anshuman Das, Mario Mietzsch, Daniel K. Oh, Jonathan Ark, Robert McKenna, Mavis Agbandje‐McKenna, Aravind Asokan
Abstract
Understanding how viruses interact with host cells through cell surface receptors is central to discovery and development of antiviral therapeutics, vaccines, and gene transfer vectors. Here, we demonstrate that distinct epitopes on the surface of adeno-associated viruses can be evolved by infectious cycling to recognize different cell surface carbohydrates and glycoprotein receptors and solve the three-dimensional structure of one such newly evolved AAV capsid, which provides a roadmap for designing viruses with improved attributes for gene therapy applications.
Topics & Concepts
BiologyTransduction (biophysics)CapsidGlycanReceptorEpitopeCell biologyComputational biologyGeneSignal transductionGenomeGlycoproteinGeneticsBiochemistryAntigenVirus-based gene therapy researchViral Infections and Immunology ResearchCRISPR and Genetic Engineering