Hyperbaric oxygen treatment impacts oxidative stress markers in patients with necrotizing soft-tissue infection
Morten Hedetoft, Peter Østrup Jensen, Claus Moser, Julie Vinkel, Ole Hyldegaard
Abstract
Necrotizing soft-tissue infection (NSTI) is a rare, severe, and fast-progressing bacterial infection associated with a high risk of developing sepsis or septic shock. Increasing evidence indicates that oxidative stress is crucial in the development and progression of sepsis, but its role in NSTI specifically has not been investigated. Some patients with NSTI receive hyperbaric oxygen (HBO 2 ) treatment as the restoration of oxidative stress balance is considered an important mechanism of action, which HBO 2 facilitates. However, a gap in knowledge exists regarding the effect of HBO 2 treatment on oxidative stress in patients with NSTI. In the present observational study, we aimed to investigate HBO 2 treatment effects on known markers of oxidative stress in patients with NSTI. We measured plasma myeloperoxidase (MPO), superoxide dismutase (SOD), heme oxygenase-1 (HO-1) and nitrite+nitrate in 80 patients with NSTI immediately before and after their first HBO 2 treatment, and on the following day. We found that HBO 2 treatment was associated with a significant increase in MPO and SOD by a median of 3.4 and 8.8 ng/mL, respectively. Moreover, we observed an HBO 2 treatment-associated increase in HO-1 in patients presenting with septic shock (n=39) by a median of 301.3 pg/mL. All markers were significantly higher in patients presenting with septic shock compared to patients without shock, and all markers correlated with disease severity. High baseline SOD was associated with 90-day mortality. In conclusion, HBO 2 treatment was associated with an increase in MPO and SOD in patients with NSTI, and oxidative stress was more pronounced in patients with septic shock.