Neutrophils inhibit CD8<sup>+</sup>T cells immune response by arginase-1 signaling in patients with sepsis
Xiaokang Dai, Zhenxing Ding, Yuanyuan Tan, Hua-rui Bao, Dongyao Wang, Hong Zhang
Abstract
BACKGROUND: Patients with sepsis often exhibit an acute inflammatory response, followed by an immunosuppressive phase with a poor immune response. However, the underlying mechanisms have not been fully elucidated. METHODS: We sought to comprehensively characterize the transcriptional changes in neutrophils of patients with sepsis by transcriptome sequencing. Additionally, we conducted a series of experiments, including real-time quantitative polymerase chain reaction (RT-qPCR) and flow cytometry to investigate the role of arginase-1 signaling in sepsis. RESULTS: T cells increased after inhibition of arginase-1 signaling. CONCLUSION: T cells could be restored by blocking arginase-1 signaling in patients with sepsis.