A Compendium of the Principal Stationary Phases Used in Hydrophilic Interaction Chromatography: Where Have We Arrived?
Maria Aurora Guarducci, Andrea Fochetti, Alessia Ciogli, Giulia Mazzoccanti
Abstract
Hydrophilic interaction liquid chromatography (HILIC) today is a well-known and largely applied technique to analyse polar compounds such as pharmaceuticals, metabolites, proteins, peptides, amino acids, oligonucleotides, and carbohydrates. Due to the large number of stationary phases employed for HILIC applications, this review aims to help the reader in choosing a proper stationary phase, which often represents the critical point for the success of a separation. A great offer is present for achiral applications in contrast to the chiral phases developed for HILIC enantioseparations. In the last case, up-to-date solutions are presented.
Topics & Concepts
Hydrophilic interaction chromatographyChemistryStationary phaseChromatographyPolarChiral stationary phaseHigh-performance liquid chromatographyAstronomyPhysicsAnalytical Chemistry and ChromatographyChromatography in Natural ProductsMicrofluidic and Capillary Electrophoresis Applications