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Utility of Polymerase Chain Reaction in Nasopharyngeal Swabs for Identifying Respiratory Bacteria Causing Community-Acquired Pneumonia

Yoris Demars, Thomas Brahier, David C. Rotzinger, René Brouillet, Katia Jaton, Onya Opota, Noémie Boillat‐Blanco

2022Microbiology Spectrum15 citationsDOIOpen Access PDF

Abstract

Timely identification of a pathogen in patients with lower respiratory tract infections is of paramount importance to avoid inappropriate antibiotic prescription. We aimed to evaluate the performance of a rapid syndromic molecular assay in nasopharyngeal swabs for identifying the most common bacterial causes of lower respiratory tract infections in adults (Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis). Our data show that nasopharyngeal molecular assay has a good concordance with lower respiratory tract sample when positive but not when negative. A positive result is therefore concordant with a lower respiratory tract infection and can be used to target antibiotics. Nevertheless, a negative result does not have a good concordance, so it cannot be used to withhold antibiotics. Our findings illustrate the potential utility of these easily collected samples for the management of patients with lower respiratory tract infections.

Topics & Concepts

Polymerase chain reactionPneumoniaMicrobiologyBacteriaRespiratory systemMedicineImmunologyBiologyVirologyInternal medicineGeneGeneticsPneumonia and Respiratory InfectionsRespiratory viral infections researchNosocomial Infections in ICU
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