Cross-validated Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Quantitation Protocol for a Pharmaceutical Drug and Its Drug-Target Effects in the Brain Using Time-of-Flight and Fourier Transform Ion Cyclotron Resonance Analyzers
Patrik Källback, Theodosia Vallianatou, Anna Nilsson, Mohammadreza Shariatgorji, Nicoletta Schintu, Marcela Pereira, Florian P.Y. Barré, Henrik Wadensten, Per Svenningsson, Per E. Andrén
Abstract
citalopram administration on the serotonin neurotransmitter system was studied in the hippocampus, a brain region that is the principal target of the serotonergic afferents along with the limbic system, and it was shown that serotonin was significantly increased (2-fold), but its metabolite 5-hydroxyindoleacetic acid was not. This study makes a substantial step toward establishing MALDI-MSI as a fully quantitative validated method.
Topics & Concepts
ChemistryFourier transform ion cyclotron resonanceMass spectrometryMass spectrometry imagingChromatographyReproducibilityIon suppression in liquid chromatography–mass spectrometryDrug detectionTandem mass spectrometryTime-of-flight mass spectrometryAnalytical Chemistry (journal)IonizationIonOrganic chemistryMass Spectrometry Techniques and ApplicationsAnalytical Chemistry and ChromatographyMetabolomics and Mass Spectrometry Studies