Cross-Genus “Boot-Up” of Synthetic Bacteriophage in Staphylococcus aureus by Using a New and Efficient DNA Transformation Method
Nacyra Assad-Garcia, Roshan D’Souza, Rachel Buzzeo, Arti Tripathi, Lauren M. Oldfield, Sanjay Vashee, Derrick E. Fouts
Abstract
phages for therapeutic applications and the engineering of Staphylococcus strains by enabling transformation of higher molecular weight DNA to introduce more complex modifications.
Topics & Concepts
Staphylococcus aureusTransformation (genetics)BacteriophageBiologyMicrobiologyDNAComputational biologyGenomeAntibioticsHuman pathogenVirologyPathogenSynthetic biologyLytic cycleBacteriagenomic DNAPhage therapyStaphylococcusVirusBacterial virusBacterial geneticsEscherichia coliPhage typingBacteriophages and microbial interactionsCancer Research and TreatmentsClick Chemistry and Applications