Contribution of the EssC ATPase to the assembly of the type 7b secretion system in Staphylococcus aureus
Maksym Bobrovskyy, So-Young Oh, Dominique Missiakas
Abstract
was required for association with EssB. This interaction was independent of EsaA, but EsaA was required to recruit EssA to the EssC-EssB complex. Finally, we show that assembly of the ESS translocon was impaired upon mutation of D2 structural motifs. Together, our data indicate that the ESS translocon is maintained fully assembled at the plasma membrane and that D2 is fundamental in sustaining the integrity of this complex.
Topics & Concepts
TransloconBiologySecretionMutantATPaseType three secretion systemCell biologyFunction (biology)CytosolGeneGeneticsBiochemistryChromosomal translocationEnzymeAntimicrobial Resistance in StaphylococcusAntibiotic Resistance in BacteriaBacterial Genetics and Biotechnology