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Divergent Total Syntheses of Elisapterane and Relevant Diterpenoids Assisted by <i>In Silico</i> Structure Reassignment

Chunhui Liu, Han Gong, Yidian Sheng, Wen-Tao Wang, Qidong Xia, Hanfeng Ding

2025Journal of the American Chemical Society8 citationsDOI

Abstract

Herein, we describe our synthetic endeavors toward elisapterane and relevant diterpenoids through a bioinspired divergent strategy based on an unprecedented late-stage D-ring formation logic. The key tricyclic intermediate bearing a norneoelisabane skeleton could be efficiently prepared via an ODI-(5 + 2) cycloaddition/1,2-acyl migration cascade and a SmI 2 -mediated pinacol coupling/Grob fragmentation/deoxygenation orchestration. The synthesis of the misassigned structure of elisapterosin F led us to revisit the structure elucidation of these natural products. Application of NMR calculation-based in silico structure reassignment disclosed several unheeded structural mutations for elisapterane diterpenoids and accurately revised the structures of elisapterosins A, D, and F. With a computation-rerouted synthetic blueprint, we eventually accomplished collective total syntheses of elisapterosins A–F, alongside biogenetically related aberrarone, elisabanolide, and 3- epi -elisabanolide. By integrating our inference aided by computational tools with validation through total synthesis, this work exemplifies a modern case of total synthesis detective stories, demonstrating the power of computer-assisted structure elucidation (CASE) and the risks of analogical approaches for the structure assignment of natural products.

Topics & Concepts

ChemistryTotal synthesisCycloadditionIn silicoStereochemistryDeoxygenationOrganic chemistryGeneBiochemistryCatalysisPlant and animal studiesTraditional and Medicinal Uses of AnnonaceaePlant biochemistry and biosynthesis