Ile258Met mutation of Brucella melitensis 7α-hydroxysteroid dehydrogenase significantly enhances catalytic efficiency, cofactor affinity, and thermostability
Zhiyong Liu, Rongzhen Zhang, Wenchi Zhang, Yan Xu
Topics & Concepts
ThermostabilityCofactorEnzyme kineticsDehydrogenaseChemistryHydroxysteroid dehydrogenaseBiochemistryNAD+ kinaseWild typeEnzymeMutantStereochemistryBiologyActive siteGeneEnzyme Catalysis and ImmobilizationSteroid Chemistry and BiochemistryCrystallization and Solubility Studies