Zwitterionic small molecule based fluorophores for efficient and selective imaging of bacterial endospores
N. Senthilnathan, Kumar Gaurav, Ch. V. Ramana, T. P. Radhakrishnan
Abstract
stains bacteria and endospores, most significantly, BHADQ stains endospores selectively. To the best of our knowledge, this is the first example of selective fluorescence imaging of endospores in their dormant state. A range of spectroscopy, microscopy and calorimetry studies provide insight into the molecular level interactions that enable efficient staining and bright images. DADQ fluorophores are photostable and non-cytotoxic, hence useful in practical applications. The versatile structural tailorability of these dye molecules holds great promise for targeted imaging.
Topics & Concepts
EndosporeMoleculeChemistrySmall moleculeBacteriaBiophysicsNanotechnologyMaterials scienceBiologyBiochemistryOrganic chemistryGeneticsLuminescence and Fluorescent MaterialsMolecular Sensors and Ion DetectionNanoplatforms for cancer theranostics