Simultaneous observation of the spatial and temporal dynamics of single enzymatic catalysis using a solid-state nanopore
Ru‐Jia Yu, Qiao Li, Shao-Chuang Liu, Hui Ma, Yi‐Lun Ying, Yi‐Tao Long
Abstract
continuous recording of ionic flux amplification. The temporal heterogeneity in enzymatic properties and its spatial dynamic orientations were observed simultaneously, and these two properties were found to be closely correlated. We anticipate that this method offers new perspectives on the correlation of protein structure and function at the single-molecule level.
Topics & Concepts
NanoporeGlucose oxidaseIonic bondingMaterials scienceMoleculeNanotechnologyChemical physicsEnzymeSolid-stateDynamics (music)Molecular dynamicsChemistryBiophysicsBiological systemComputational chemistryPhysical chemistryBiosensorIonPhysicsBiochemistryOrganic chemistryBiologyAcousticsNanopore and Nanochannel Transport StudiesElectrochemical Analysis and ApplicationsMicrofluidic and Capillary Electrophoresis Applications