First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora
Ismahen Akremi, Dominique Holtappels, Wided Brabra, Mouna Jlidi, Adel HADJ BRAHIM, Manel Ben Ali, Kiandro Fortuna, Mohammed H. Ahmed, Bart Van Meerbeek, Ali Rhouma, Rob Lavigne, Mamdouh Ben Ali, Jeroen Wagemans
Abstract
Newly discovered Erwinia amylovora phages PEar1, PEar2, PEar4 and PEar6 were isolated from three different orchards in North Tunisia to study their potential as biocontrol agents. Illumina sequencing revealed that the PEar viruses carry a single-strand DNA genome between 6608 and 6801 nucleotides and belong to the Inoviridae, making them the first described filamentous phages of E. amylovora. Interestingly, phage-infected cells show a decreased swimming and swarming motility and a cocktail of the four phages can significantly reduce infection of E. amylovora in a pear bioassay, potentially making them suitable candidates for phage biocontrol.