Controllable Fabrication of Composite Core–Shell Capsules at a Macroscale as Organoid Biocarriers
Fan He, Tingting Tao, Haitao Liu, Yaqing Wang, Kangli Cui, Yaqiong Guo, Jianhua Qin
Abstract
electrostatic interactions, a PEI polyelectrolyte can be incorporated in the shell for restricting its swelling, thus effectively improving the stability of the capsules. The Alg/PEI macrocapsules are featured with desirable selective permeability for immunoisolation of antibodies from reaching the loaded organoids. Meanwhile, they also exhibit excellent permeability for mass transfer due to their well-defined core-shell structure. As such, the encapsulated islet organoids contain islet-specific multicellular components, with high viability and sensitive glucose-stimulated insulin secretion function. The proposed approach provides a versatile encapsulation system for tissue engineering and regenerative medicine applications.