Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
Richard Patryk Ngondo, Michel Cohen‐Tannoudji, Constance Ciaudo
Abstract
Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn. For complete details on the use and execution of this protocol, please refer to Ngondo et al. (2018).
Topics & Concepts
Embryoid bodyEndodermGerm layerEmbryonic stem cellCell biologyBlastocystBiologyStem cellIn vitroCellular differentiationEmbryogenesisEmbryoAdult stem cellGeneticsInduced pluripotent stem cellGenePluripotent Stem Cells ResearchCRISPR and Genetic EngineeringRenal and related cancers