Dinoroseobacter shibae Outer Membrane Vesicles Are Enriched for the Chromosome Dimer Resolution Site <i>dif</i>
Hui Wang, Nicole Beier, Christian Boedeker, Helena Sztajer, Petra Henke, Meina Neumann‐Schaal, Johannes Mansky, Manfred Rohde, Jörg Overmann, Jörn Petersen, Frank Klawonn, Martin Kucklick, Susanne Engelmann, Jürgen Tomasch, Irene Wagner‐Döbler
Abstract
, a symbiont of dinoflagellates, using microscopy and a multi-omics approach. We found that vesicles formed during undisturbed exponential growth contain DNA which is enriched for genes around the replication terminus, specifically, the binding site for an enzyme complex that is activated at the last stage of cell division. We suggest that the enriched genes are the result of overreplication which is repaired by their excision and excretion via membrane vesicles to clear the divisome from waste DNA.
Topics & Concepts
Bacterial outer membraneVesicleDimerCytoplasmBiophysicsDNAMembraneBiologyChemistryCell biologyBiochemistryEscherichia coliGeneOrganic chemistryBacterial Infections and VaccinesLegionella and Acanthamoeba researchProtist diversity and phylogeny