A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging
Ming‐Xuan Hou, Liu‐Yi Liu, Kang‐Nan Wang, Xi‐Juan Chao, Rong-Xue Liu, Zong‐Wan Mao
Abstract
A two-photon fluorescent probe was developed for detecting mitochondrial viscosity during apoptosis of living cells by two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM) with good selectivity and highly biocompatible.
Topics & Concepts
ChemistryFluorescenceFluorescence microscopeTwo-photon excitation microscopyFluorescence-lifetime imaging microscopyMicroscopyRotor (electric)ApoptosisBiophysicsViscosityMitochondrionNuclear magnetic resonanceNanotechnologyOpticsBiochemistryPhysicsMaterials scienceBiologyQuantum mechanicsMolecular Sensors and Ion DetectionLipid Membrane Structure and BehaviorPorphyrin and Phthalocyanine Chemistry