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Exploring a solid-state nanopore approach for single-molecule protein detection from single cells

Ziqi Zhou, Shao-Chuang Liu, Jia Wang, Kele Chen, Bao-Kang Xie, Yi‐Lun Ying, Yi-Tao Long

2025Chemical Science22 citationsDOIOpen Access PDF

Abstract

nanopores. Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples. This work demonstrates the potential of solid-state nanopores as a powerful tool for single-cell, single-molecule protein analysis, opening avenues for investigating protein dynamics and interactions at the cellular level.

Topics & Concepts

NanoporeSolid-stateMoleculeSingle useState (computer science)Single-cell analysisChemistryNanotechnologyComputer scienceMaterials scienceBiochemistryEngineeringCellPhysical chemistryAlgorithmOrganic chemistryProcess engineeringNanopore and Nanochannel Transport StudiesAdvanced biosensing and bioanalysis techniquesIon-surface interactions and analysis