A highly sensitive signal-on biosensor for microRNA 142-3p based on the quenching of Ru(bpy)<sub>3</sub><sup>2+</sup>–TPA electrochemiluminescence by carbon dots and duplex specific nuclease-assisted target recycling amplification
Yang Jiao, Qian Xia, Longhua Guo, Fang Luo, Yongqiang Dong, Bin Qiu, Zhenyu Lin
Abstract
Carbon dots (CDs) have been used to quench the electrochemiluminescence (ECL) of a Ru(bpy)32+-tripropylamine (TPA) system for the first time. In addition, this phenomenon has been coupled with duplex specific nuclease-assisted target recycling amplification to develop a highly sensitive ECL biosensor for microRNA.
Topics & Concepts
ElectrochemiluminescenceNucleaseBiosensorQuenching (fluorescence)Duplex (building)ChemistrymicroRNACombinatorial chemistryFluorescenceMaterials scienceNanotechnologyPhotochemistryDNAElectrodePhysicsBiochemistryPhysical chemistryGeneQuantum mechanicsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryMicroRNA in disease regulation