Assembly of a Nanogold-Assisted Aptamer Sensor for Highly Sensitive Detection of Homocysteine
Shaoshuai Wang, Yingzi Mi, Guoyong Ren, Yanjun Chen, Xuemei Wu
Abstract
With more in-depth exploration on stroke risk factors in recent years, an increasing number of studies show that homocysteine (HCY) is a new risk factor of cerebral infarction. In this work, graphene was adopted for the modification of glassy carbon electrodes, and gold nanoparticles were modified onto the graphene surface with a simple and controlled electrodeposition method, followed by aptamer immobilization, to construct the homocysteine electrochemical sensor. Under optimum conditions, the analytical performance of the proposed aptamer sensor showed a positive response to different concentrations of HCY. The peak currents were proportional to HCY concentration. The linear regression equation can be found from 0.05–20.0 μM. The limit of detection can be calculated to be 5 nM based on S/N=3.