Xenometabolite signatures in the UC Davis type 2 diabetes mellitus rat model revealed using a metabolomics platform enriched with microbe-derived metabolites
Kelly E. Mercer, Laxmi Yeruva, Lindsay Pack, James L. Graham, Kimber L. Stanhope, Sree V. Chintapalli, Umesh D. Wankhade, Kartik Shankar, Peter J. Havel, Sean H. Adams, Brian D. Piccolo
Abstract
We debut a liquid chromatography-mass spectrometry (LC/MS) platform called the XenoScan, which is a metabolomics platform for xenometabolites (nonself-originating metabolites). This assay has 190 in-house standards with the majority enriched for microbe-derived metabolites. As a proof-of-principle, we used the XenoScan to discriminate genetic differences from cecal samples associated with different rat lineages, in addition to characterizing diabetes progression in rat model of type 2 diabetes. Complementing microbial sequencing data with xenometabolites uncovered novel microbial metabolism in targeted organisms.