A nanoprobe for fluorescent monitoring of microRNA and targeted delivery of drugs
Zuo Chen, Yongcan Guo, Junjie Li, Zhiping Peng, Shulian Bai, Shuangshuang Yang, Ding Wang, Hui Chen, Guoming Xie
Abstract
ligand exchange with phosphates. In addition, the nanoprobe showed good selectivity, a linear response towards miR-21 ranging from 4 nM to 500 nM, and a limit of detection in 4 nM, which precluded unintended payload leakage due to low-abundance endogenous miR-21 expression in normal cells. Moreover, based on a dual-targeted delivery system constituted by AS1411-mediated recognition and responsive release of DOX, a specific cytotoxic efficacy was observed in MCF-7 cells. The present work provides a smart and robust nanoprobe for real-time detection of miRNA and dual-responsive drug delivery in tumor cells.
Topics & Concepts
NanoprobemicroRNAFluorescenceNanotechnologyChemistryPharmacologyCancer researchMedicineMaterials scienceBiochemistryNanoparticlePhysicsGeneQuantum mechanicsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryNanoplatforms for cancer theranostics