Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region
Dahae Hailey Bae, Michael Marino, Brian J. Iaffaldano, Sydney Fenstermaker, Sandra Afione, Takele Argaw, Jacob McCright, Anna Kwilas, John A. Chiorini, Andrew E. Timmons, Jakob Reiser
Abstract
TU/mL. The capacity of the T antigen-negative cells to produce high titer adeno-associated virus (AAV) vectors was also evaluated. The results obtained revealed that the lack of T antigen sequences did not impact AAV vector titers.
Topics & Concepts
HEK 293 cellsComputational biologyVector (molecular biology)AntigenCoding regionCoding (social sciences)BiologyMolecular biologyGeneticsComputer scienceVirologyGeneRecombinant DNAMathematicsStatisticsCAR-T cell therapy researchVirus-based gene therapy researchViral Infectious Diseases and Gene Expression in Insects