Kinetics of H2O2-driven catalysis by a lytic polysaccharide monooxygenase from the fungus Trichoderma reesei
Silja Kuusk, Priit Väljamäe
Abstract
in the ascorbic acid peroxidase reaction followed the ping-pong mechanism and led to irreversible inactivation of the enzyme with a probability of 0.0072. Using theoretical analysis, we suggest a relationship between the half-life of LPMO, the values of kinetic parameters, and the concentrations of the reactants.
Topics & Concepts
Trichoderma reeseiCelluloseChemistryGlycosidic bondAscorbic acidMonooxygenasePeroxidasePolysaccharideCatalysisEnzyme kineticsSubstrate (aquarium)StereochemistryCellulaseEnzymeOrganic chemistryActive siteCytochrome P450Food scienceGeologyOceanographyEnzyme-mediated dye degradationBiofuel production and bioconversionEnzyme Catalysis and Immobilization