Choroid plexus-mediated CSF secretion remains stable in aging rats via high and age-resistant metabolic activity
Sara Diana Lolansen, E. Révész, Søren Norge Andreassen, Marleen Trapp, Chiara Salio, Marco Sassoé‐Pognetto, Jens V. Andersen, Emil W. Westi, Trine L. Toft‐Bertelsen, Jonathan H. Wardman, Anne-Kristine Meinild Lundby, Flemming Dela, Annarita Patrizi, Blanca I. Aldana, Nanna MacAulay
Abstract
Our brain is bathed in cerebrospinal fluid (CSF) that is produced by the choroid plexus. CSF serves as a dispersion route for hormones and nutrients, and a conduit for waste clearance. Age-dependent reduction in the CSF secretion rate could influence cerebral waste clearance and thereby promote cognitive deficits in the elderly. Here, we resolve age-dependent modulation of CSF dynamics and choroid plexus function by complementary in vivo determinations of intracranial pressure (ICP) and CSF secretion/absorption rates, combined with transcriptomic, morphological, and metabolic analysis of choroid plexus in aging male rats. ICP and CSF secretion rate and absorption capacity remain stable with age, and the choroid plexus retains its morphology, structural integrity, gene expression, and high metabolic rate across the tested ages. This work supports the significance of choroid plexus function for brain aging and promotes this tissue as a future target for neurodegenerative diseases associated with impaired waste clearance and cognitive decline. Choroid plexus produces the cerebrospinal fluid (CSF) that serves as the brain dispersion route for nutrients and waste products. Here, the authors show that CSF secretion remains high in aging rats with intact choroid plexus morphology, gene expression, and high metabolic rate.