An Activatable NIR Probe for the Detection and Elimination of Senescent Cells
Yang Liu, Guopan Liu, Qingxin Chen, Yingpeng Wan, Zhiyang Liu, Jie Zhang, Chen Huang, Zhi‐Qiang Xu, Shengliang Li, Chun‐Sing Lee, Liang Zhang, Hongyan Sun
Abstract
Cellular senescence is involved in diverse physiological processes. Accumulation of senescent cells can lead to numerous age-related diseases. Therefore, it is of great significance to develop chemical tools to effectively detect and eliminate senescent cells. Till date, a dual functional probe that could detect and eliminate senescent cells has yet been accomplished. Herein, a β-gal-activated probe, MB-βgal, based on the methylene blue (MB) fluorophore, was designed to detect and eliminate senescent cells. In the absence of β-gal, the probe showed no fluorescence and its 1O2 production efficiency was suppressed simultaneously. On the other hand, MB-βgal could be specifically activated by the high level of β-gal in senescent cells, thus, releasing free MB with near-infrared (NIR) fluorescence and high 1O2 production efficiency under light irradiation. MB-βgal demonstrated a fast response, high sensitivity, and high selectivity in detecting β-gal in an aqueous solution and was further applied to visualization and ablation of senescent cells. As a proof of concept, the dual functions of MB-βgal were successfully demonstrated in senescent HeLa cells and mouse embryonic fibroblast cells.