Extraction, Screening and Spectral Characterization of Tanninsfrom Acacia Xanthophloea (Fever Tree) Bark
Ramesh Duraisamy, Tesema Shuge, Bezawit Worku, Abiyu Kerebo Berekete, Karthikeyan M Ramasamy
Abstract
Identifying studied sample acacia species selected and collected from Bekele Molla Hotel Avenue, Chamo Kebele, Arba Minch under convenient random sampling method. Tannins extracted using the solvents such as water (at 60 & 900C), methanol (at 900C) and its 1:1 mixture (at 900C) by employing Soxhlet extraction. Extracted crude tannins was separated and purified under vacuum distillation. The photochemical screening and quantitative determinations of sample carried out. Furthermore, the sample of tannins characterized by employing U-visible and FTIR spectroscopic studies. Methonalic extract consist moisture content and % yield about 17.86±0.51 % and 52.55 %. The results of Uv-visible and FTIR spectroscopic studies were supporting that the sample of Acacia xanthophloea has tannins and flavonoids. Thus, this study reveals and confirmed that the methanol is more suitable solvent for extraction of tannins over than water