Brief guide to western blot assays
Seokjun G. Ha, Ji-Hoon Park, Mi-Young Kim, Seung‐Jae Lee
Abstract
Western blot is an essential method that detects specific proteins using antibodies, which is one of the most widely applied techniques for protein detection. Here, we present a brief guide to western blot, following the overall procedure from the choice of antibodies to quantification. This guide will provide useful information for researchers who are unfamiliar with western blot assays.
Topics & Concepts
Western blotBlotAntibodyBiologyMolecular biologyWestern europeComputational biologyDot blotNorthern blotProtein expressionVirologyCancer Research and TreatmentsToxoplasma gondii Research StudiesAdvanced Proteomics Techniques and Applications