Generating and Utilizing Murine Cas9-Expressing Intestinal Organoids for Large-Scale Knockout Genetic Screening
Hossein Kashfi, Nicholas Jinks, Abdolrahman S. Nateri
Topics & Concepts
OrganoidCRISPRBiologyMatrigelGene knockoutCell biologyCas9Induced pluripotent stem cellCarcinogenesisGeneticsCell cultureGeneEmbryonic stem cellCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchSingle-cell and spatial transcriptomics