CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes
J. Armando Casas-Mollano, Matthew H. Zinselmeier, Samuel E. Erickson, Michael J. Smanski
Abstract
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans . Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.
Topics & Concepts
CRISPRComputational biologyGeneGenome editingGene expressionGenome engineeringBiologyRegulation of gene expressionSynthetic biologyGeneticsCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsAnimal Genetics and Reproduction