Taylor dispersion analysis and release studies of β-carotene-loaded PLGA nanoparticles and liposomes in simulated gastrointestinal fluids
Roman M. Fortunatus, Sandor Balog, Flávia Sousa, Dimitri Vanhecke, Barbara Rothen‐Rutishauser, Patricia Taladriz‐Blanco, Alke Petri‐Fink
Abstract
-glycolic acid) (PLGA) NPs and liposomes before and after exposure to simulated gastrointestinal fluids using various methods such as Taylor dispersion analysis (TDA), cryo-transmission electron microscopy, dynamic light scattering (DLS), and nanoparticle tracking analysis (NTA). TDA, a microfluidic technique, proved more effective than DLS and NTA in determining nanoparticle size in simulated gastrointestinal fluids. This highlights TDA's potential for assessing nanoparticle colloidal stability in simulated gastro-intestinal fluids, crucial for evaluating encapsulated bioactives' bioavailability. High-performance liquid chromatography (HPLC) revealed that PLGA nanoparticles incorporate and preserve βC more effectively during long-term storage compared to liposomes. Adding ascorbic acid significantly reduced degradation in simulated gastrointestinal fluids. Release studies showed that liposomes released 52% of βC after 36 hours, while PLGA nanoparticles released only 9% over 168 hours. These results provide valuable insights for selecting an appropriate βC nanocarrier for oral delivery based on desired release rates.