Construction of single, double, or triple mutants within kojic acid synthesis genes kojA, kojR, and kojT by the CRISPR/Cas9 tool in Aspergillus oryzae
Yuzhen Li, Huanxin Zhang, Ziming Chen, Junxia Fan, Tianming Chen, Bin Zeng, Zhe Zhang
Topics & Concepts
Kojic acidCRISPRMutantAspergillus oryzaeGenome editingGeneBiologyCas9MultiplexSelectable markerMutagenesisGeneticsComputational biologyPlasmidBiochemistryTyrosinaseEnzymeCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsInsect symbiosis and bacterial influences