Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes
Juan Chen, Jinlu Tang, Hong‐Min Meng, Zhuo Liu, Lin Wang, Xin Geng, Yanan Wu, Lingbo Qu, Zhaohui Li
Abstract
Here, we develop a facile split aptamer-based system for the amplified, specific and wash-free detection of exosomes in situ assisted by a target-induced hybridization chain reaction (HCR). This design was successfully used to detect target exosomes in a bio-matrix and distinguish cancer patients from healthy individuals.
Topics & Concepts
MicrovesiclesAptamerChain reactionComputational biologyMolecular biologyChemistryIn situBiologymicroRNAGeneGeneticsOrganic chemistryPhotochemistryAdvanced biosensing and bioanalysis techniquesExtracellular vesicles in diseaseRNA Interference and Gene Delivery