Evaluation of next generation sequencing approaches for SARS-CoV-2
Valentina Curini, Massimo Ancora, Lucija Jurišić, Valeria Di Lollo, Barbara Secondini, Luana Fiorella Mincarelli, M. Caporale, Ilaria Puglia, Luigina Di Gialleonardo, Iolanda Mangone, Marco Di Domenico, Adriano Di Pasquale, Alessio Lorusso, Maurilia Marcacci, Cesare Cammà
Abstract
Within public health control strategies for SARS-CoV-2, whole genome sequencing (WGS) is essential for tracking viral spread and monitoring the emergence of variants which may impair the effectiveness of vaccines, diagnostic methods, and therapeutics. In this manuscript different strategies for SARS-CoV-2 WGS including metagenomic shotgun (SG), library enrichment by myBaits® Expert Virus-SARS-CoV-2 (Arbor Biosciences), nCoV-2019 sequencing protocol, ampliseq approach by Swift Amplicon® SARS-CoV-2 Panel kit (Swift Biosciences), and Illumina COVIDSeq Test (Illumina Inc.), were evaluated in order to identify the best approach in terms of results, labour, and costs. The analysis revealed that Illumina COVIDSeq Test (Illumina Inc.) is the best choice for a cost-effective, time-consuming production of consensus sequences.