Functionalization of colloidal nanoparticles with a discrete number of ligands based on a “HALO-bioclick” reaction
Stefania Garbujo, Elisabetta Galbiati, Lucia Salvioni, Matteo Mazzucchelli, Gianni Frascotti, Xing Sun, Saad Megahed, Neus Feliu, Davide Prosperi, Wolfgang J. Parak, Miriam Colombo
Abstract
A recombinant HALO-GFP fusion protein was designed and isolated to demonstrate the feasibility of controlling the number and orientation of protein ligands to be conjugated on colloidal gold nanoparticles. AuNPs functionalized with exactly one or exactly two GFP molecules exhibited fully preserved functionality of the protein. The method is very straightforward and generally provides highly bioactive nanoparticle-protein conjugates.
Topics & Concepts
Surface modificationConjugated systemHaloColloidal goldColloidNanoparticleChemistryRecombinant DNANanotechnologyCombinatorial chemistryBiophysicsMaterials sciencePolymerOrganic chemistryBiochemistryBiologyPhysicsPhysical chemistryGalaxyGeneQuantum mechanicsGold and Silver Nanoparticles Synthesis and ApplicationsNanoparticle-Based Drug DeliveryAdvanced biosensing and bioanalysis techniques