High-Throughput Screening Method for Directed Evolution and Characterization of Aldol Activity of D-Threonine Aldolase
Lei Gong, Guochao Xu, Xudong Cao, Ruizhi Han, Jinjun Dong, Ye Ni
Topics & Concepts
Aldol reactionAldolase AChemistryDirected evolutionThreonineSaturated mutagenesisHigh-throughput screeningAldol condensationStereochemistryBiochemistryOrganic chemistryChromatographyCatalysisEnzymeSerineMutantGeneAmino Acid Enzymes and MetabolismEnzyme Catalysis and ImmobilizationEnzyme Structure and Function