Insights into the fungal secretomes and their roles in the formation and stabilization of the biogenic silver nanoparticles
Thyerre Santana da Costa, Gonzalo García Delgado, Carolyne B. Braga, Ljubica Tasić
Abstract
, as demonstrated through bacterial growth inhibition assays. The presence of redox proteins, such as glyceraldehyde reductase and FAD-oxidoreductase, suggests a potential mechanism for the generation of reactive oxygen species (ROS) and oxidative stress in bacterial cells, further validated by fluorescence microscopy to differentiate viable from non-viable cells. Unlike previous studies that have focused separately on metal ion reduction or nanoparticle stabilization, our findings reveal a coordinated biosynthetic process where the same proteins mediate both functions. This overlap between the secretome and nanoparticle proteome provides new insights into fungal-mediated nanoparticle synthesis, highlighting the multifunctionality of fungal proteins in bionanotechnology. By demonstrating how secreted enzymes directly contribute to nanoparticle formation, this study paves the way for more efficient, scalable, and environmentally sustainable approaches to biogenic nanoparticle production.