A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease
André A. Teixeira, Luis Rodriguez Carnero, Andréia Kuramoto, Fenny H. F. Tang, Carlos Hernique Gomes, Natalia Bueno Pereira, Léa Campos de Oliveira, Regina Garrini, Jhonatas Sirino Monteiro, João Carlos Setúbal, Éster Cerdeira Sabino, Renata Pasqualini, Walter Colli, Wadih Arap, Maria Júlia Manso Alves, Edécio Cunha‐Neto, Ricardo J. Giordano
Abstract
enable the identification of thousands of antigens recognized by serum samples from patients with Chagas disease. Because most of these antigens are hypothetical proteins, gPhage provides evidence of their expression during infection. We built and validated a comprehensive map of Chagas disease antibody response to show how linear and putative conformation epitopes, many rich in repetitive elements, allow the parasite to evade a buildup of neutralizing antibodies directed against protein domains that mediate infection pathogenesis. Thus, the gPhage platform is a reproducible and effective tool for rapid simultaneous identification of epitopes and antigens, not only in Chagas disease but perhaps also in globally emerging/reemerging acute pathogens.