Precisely Structured Nitric-Oxide-Releasing Copolymer Brush Defeats Broad-Spectrum Catheter-Associated Biofilm Infections <i>In Vivo</i>
Zheng Hou, Yang Wu, Chen Xu, Sheethal Reghu, Zifang Shang, Jingjie Chen, Dicky Pranantyo, Kalisvar Marimuth, Partha Pratim De, Oon Tek Ng, Kévin Pethe, E. T. Kang, Peng Li, Mary B. Chan‐Park
Abstract
-S)) shows unprecedented, above 99.99%, in vitro biofilm inhibition of Gram-positive and Gram-negative bacteria, 100-fold better than previous coatings. It has negligible toxicity toward mammalian cells and excellent blood compatibility. In a murine subcutaneous infection model, it achieves >99.99% biofilm reduction of Gram-positive and Gram-negative bacteria compared with <90% for silver catheter, while in a porcine central venous catheter infection model, it achieves >99.99% reduction of MRSA with 5-day implantation. This precision coating is readily applicable for long-term biofilm-resistant and blood-compatible copolymer coatings covalently grafted from a wide range of medical devices.