CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
Changyang Zhou, Xinde Hu, Cheng Tang, Wenjia Liu, Shaoran Wang, Yingsi Zhou, Qimeng Zhao, Qiyu Bo, Linyu Shi, Xiaodong Sun, Haibo Zhou, Hui Yang
Abstract
RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo could prevent the development of laser-induced CNV in mouse model of Age-related macular degeneration.
Topics & Concepts
Choroidal neovascularizationMacular degenerationGene knockdownIn vivoRNAIn vitroBiologySmall hairpin RNAOphthalmologyCell biologyCancer researchMedicineGeneticsGeneCRISPR and Genetic EngineeringRetinal Development and DisordersRNA regulation and disease