Proof of concept of using a membrane-sensing peptide for sEVs affinity-based isolation
Beatriz Benayas, Joaquín Morales, Alessandro Gori, Alessandro Strada, Paola Gagni, Roberto Frigerio, Carolina Egea, Pilar Armisén, Marina Cretich, María Yáñez‐Mó
Abstract
This new isolation methodology is based on the recognition of general membrane characteristics independent of surface markers. It is thus unbiased and can be used in any species EV sample, even in samples from animal or plant species against which no suitable antibodies exist. Being an affinity method, the sample handling protocol is very simple, less time-consuming, does not require specialized equipment and can be easily introduced in a clinical automated routine. We demonstrated the high purity and yield of the method in comparison with other commercially available kits. This method can also be scale up or down, with the possibility of analyzing very low amounts of sample, and it is compatible with any downstream analyses thanks to the gentle elution procedure.