In vitro and in silico assessment of antidiabetic and antioxidant potencies of secondary metabolites from Gymnema sylvestre
Yves Oscar Nganso Ditchou, Peron Bosco Leutcha, David Miaffo, Mamoudou Hamadou, Mohd Sajid Ali, Gabrielle Ange Amang à Ngnoung, Désiré Soh, Mohit Agrawal, Rosalie Darbawa, Ernestine Zondegoumba Nkwengoua Tchouboun, Alain Méli Lannang, Xavier Siwe Noundou
Abstract
= 218.46 and 57.42 µg/mL for α-glucosidase and α-amylase respectively) suggesting its potential for managing postprandial hyperglycaemia. In silico studies employed molecular docking and dynamics simulations to elucidate the interactions between identified compounds and α-amylase/α-glucosidase enzymes. The results revealed promising binding affinities between the compounds and target enzymes, with compound 6 demonstrating the highest predicted inhibitory activity with -10 kcal/mol and -9.1 kcal/mol for α-amylase and α-glucosidase, respectively. This study highlights the presence of diverse bioactive compounds in Gymnema sylvestre. The extract exhibits antioxidant properties and inhibits carbohydrate-digesting enzymes, suggesting its potential as a complementary therapeutic approach for managing hyperglycaemia associated with type 2 diabetes.