Structure Analysis and Antioxidant Activity of a Novel Polysaccharide from Katan Seeds
Imen Trabelsi, Sirine Ben Slima, Naourez Ktari, Mohamed Bouaziz, Riadh Ben Salah
Abstract
In the present work, a novel water‐soluble polysaccharide (LWSP) was purified from Katan seeds. Polysaccharide was structurally characterized by NMR spectroscopic analysis, thin‐layer chromatography (TLC), high‐performance liquid chromatography (HPLC), Fourier‐transform infrared spectroscopy (FTIR) analysis, X‐ray diffraction (XRD), and UV absorption. TLC and HPLC showed that LWSP was a polysaccharide consisted mainly of glucose, mannose, xylose, and arabinose. The FTIR spectrum and UV absorption proved polysaccharide characteristic of LWSP. According to XRD, LWSP presented a semicrystalline behavior. The molecular weight was estimated as 64.56 kDa. Results obtained through 13 C and 1 H nuclear magnetic resonance (NMR) indicated that LWSP is consisted of four monosaccharide residues with α and β anomers. Physicochemical and antioxidant properties of LWSP were also investigated. Results revealed that LWSP exhibited interesting 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) (IC 50 = 4.48 mg/ml) and chelating activity (IC 50 = 4.79 mg/ml), and it displayed moderate reductive capacities. Overall, the findings suggested that LWSP is a promising source of natural additives in various industries fields.