Gene editing to induce FOXP3 expression in human CD4 <sup>+</sup> T cells leads to a stable regulatory phenotype and function
Yuchi Honaker, Nicholas Hubbard, Yufei Xiang, Logan Fisher, David Hagin, Karen Sommer, Yumei Song, Soo Jung Yang, Christina Lopez, Tori Tappen, Elizabeth M. Dam, Iram Khan, Malika Hale, Jane H. Buckner, Andrew M. Scharenberg, Troy R. Torgerson, David J. Rawlings
Abstract
Gene editing to insert a strong promoter upstream of endogenous FOXP3 conferred T reg -like properties and functions to primary human CD4 + T cells.
Topics & Concepts
PhenotypeFunction (biology)GeneEndogenyBiologyFOXP3Gene expressionGenome editingRegulation of gene expressionGeneticsCell biologyMolecular biologyImmune systemCRISPRBiochemistryImmune Cell Function and InteractionT-cell and B-cell ImmunologyCytomegalovirus and herpesvirus research