Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis
Joshua C. Corpuz, Larissa M. Podust, Tony D. Davis, M. Jaremko, Michael D. Burkart
Abstract
Using a covalent chemical probe and X-ray crystallography coupled to nuclear magnetic resonance data, we elucidated the dynamic molecular basis of protein recognition between the carrier protein and adenylation domain in pyoluteorin biosynthesis. These findings reveal a unique binding mode, which contrasts previously solved carrier protein and partner protein interfaces.
Topics & Concepts
BiosynthesisVisualizationComputer scienceComputational biologyChemistryBiologyBiochemistryArtificial intelligenceEnzymeMicrobial Natural Products and BiosynthesisGlycosylation and Glycoproteins ResearchGenomics and Phylogenetic Studies